Thirty-five female patients with chronic ankle instability were randomized to receive 6 weeks of Single-Limb Balance Training exercises performed on the non-affected side (n=12), 6 weeks of Single-Limb Balance Training exercises performed on the affected side (n=12), or no treatment (n=11). The outcomes of interest included Mediolateral Stability Index (MLSI), Antero-Posterior Stability Index (APSI), and the Overall Stability Index (OASI), all measured before and after training. Results revealed statistically significant improvements from baseline in all outcomes in both the cross-training group and traditional training group (p<0.05 for all). In addition, OASI and MLSI scores were statistically significantly in favour of the cross-training group compared to the control group (p<0.05 for both). Also, OASI, APSI, and MLSI scores were statistically significantly in favour of the traditional training group compared to the control group (p<0.05 for all). No statistical differences were observed between the cross training and traditional training groups in all outcomes (p>0.05 for all).