Seventy-three young adults with chronic ankle instability were randomized to receive either uphill running combined with joint mobilization (URJM; n=18), uphill running alone (UR; n=18), joint mobilization alone (JM; n=17), or no intervention (control; n=23). The primary outcomes of interest were the Cumberland Ankle Instability Tool (CAIT) score and the Y-balance test (YBT) in three directions. Secondary outcomes included the weight-bearing lunge test (WBLT) and the non-weight-bearing dorsiflexion range of motion (NWBG). Outcomes were assessed at baseline and after 4 weeks. Overall, the results revealed that all intervention groups improved in CAIT and dynamic stability compared to controls, with the URJM group showing the largest improvement in dorsiflexion. This suggests that combining uphill running and joint mobilization can better enhance ankle mobility than either intervention alone.